splB [2019-07-31 13:40:46]

spore photoproduct lyase, radical SAM enzyme

Locus: BSU_13930

Isoelectric point: 8.52

Molecular weight: 39.79 kDa

Protein length: 342 aa

Gene length: 1029 bp

Function: protection of spore DNA against photodamage

Product: spore photoproduct lyase

Essential: no

Synonyms: spl

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 1,461,770 → 1,462,798

Phenotypes of a mutant

• sensitive to blue light-induced DNA damage PubMed

The protein

Catalyzed reaction/ biological activity

• repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase

Protein family

• Radical SAM superfamily (according to UniProt)

Cofactors

• Fe-S cluster PubMed
• uses the [4Fe-4S] cluster to reduce SAM

Structure

• 4FHC (SplB from Geobacillus thermodenitrificans, 72% identity, 90% similarity) PubMed

Expression and Regulation

Operons

• Genes: splA-splB
  Description: PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon

  Regulatory mechanism

  • SplA: negative autoregulation, in SplA regulon

  Regulation

  • expressed during sporulation in the forespore (SigG) PubMed

Biological materials

Mutant

• BP130 (del splB::spc), available in Fabian Commichau's lab
• BKE13930 (ΔsplB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATGCCACATCCTTTTC, downstream forward: _UP4_TTCACTTAAACGGGCTGTTG
• BKK13930 (ΔsplB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATGCCACATCCTTTTC, downstream forward: _UP4_TTCACTTAAACGGGCTGTTG

References

Reviews

Loading

Structure of the spore photoproduct lesion in DNA

Loading

Original publications

Loading